Journal: Cell Death & Disease
Article Title: The deubiquitination enzyme USP14 promotes the tumourigenesis of gastric cancer by enhancing c-MYC nuclear translocation through deubiquitination of KPNA2
doi: 10.1038/s41419-025-08065-2
Figure Lengend Snippet: A Box plot of USP14 expression levels in the peritumoral tissues (normal) and GC tumors with log-rank test P values < 0.05. B , C Kaplan–Meier analysis of progression-free survival using data from the R2 database and Kaplan-Meier Plotter database. D , E qRT-PCR and Western blot assays were used to detect the expression of USP14 in the human normal gastric cell line (GES-1) and GC cell lines (MKN-45, MGC-803, BGC-823, SGC-7901, HGC-27). F Immunohistochemical staining analysis showed the expression of USP14 in different stages of gastric cancer tissues. Scale bar(black) = 200 μm. Scale bar(red) = 50 μm. All data are expressed as the mean ± SD. Student’s t test was performed to analyze significance. * P < 0.05, ** P < 0.01, *** P < 0.001.
Article Snippet: All human GC cell lines (BGC-823, HGC-27, MGC-803, MKN-45, and SGC-7901), Human normal gastric cell line (GES-1), and human embryonic renal cell line HEK293FT were obtained from the American Type Culture Collection (ATCC, Beijing, China).
Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemical staining, Staining